Introduction: Brain damage is often irreversible due to poor brain's self-repairing ability. New treatment strategies focused on stem cell therapy and 3-dimension matrix for brain tissue injury. The extracellular matrix (ECM) of animal tissues is a complex mixture of macromolecules that play an essential instructional role in the development of tissues and organs. Therefore, tissue engineering approaches rely on the need to present the correct cues to cells and to guide them to maintain tissue-specific functions. Recent research efforts on ECM have showed various sequences and motifs, which play key roles in improvement of the brain function after injury. Conclusion: Small motif from ECM molecules can mimic some of the biological functions of their large molecules. Peptides sequences and motifs laminin can be linked to various biomaterials scaffolds and provide the cells with mechanical support. This may ensure appropriate cell growth that aids the formation of the correct tissue structure.